Roche公司研发出适用于实时定量PCR检测的产品

 
 
即配即用试剂混合物，简化了定量PCR（qPCR）以及两步法定量逆转录PCR(qRT-PCR)的样品准备工作。

FastStart TaqMan探针试剂盒和FastStart SYBR绿试剂盒混合物最适合用于大多数实时PCR设备，除了LightCycler2.0设备。具有两种形式，一种包含ROX参考染料，另一种则不包含。联合使用实时PCR设备、合适的PCR引物以及水解探针，FastStart TaqMan探针试剂盒可以进行非常敏感的检测和定量特定DNA序列。

FastStart TaqMan探针试剂盒与Roche诊断学公司的通用探针文库的探针能够很好的工作，正是有Roche公司研发了这种产品。

FastStart SYBR绿试剂盒是一种即配即用的双浓缩混合物，包含所有必须的试剂（除了引物和模板），适合在qPCR和两步法qRT-PCR分析的时候，采用SYBR Green I模式的检测，进行定量实时DNA检测分析。理论上说：FastStart TaqMan探针试剂盒和FastStart SYBR绿试剂盒能够用于任何DNA或者cDNA的扩增与检测。

Master Mixes for PCR Instruments
 
Ready-to-use reagent mixes simplify the preparation of samples for quantitative polymerase chain reaction (qPCR) and two-step quantitative reverse transcription PCR (qRT-PCR).

The FastStart TaqMan Probe Master and FastStart SYBR Green Master mixes are optimized for use with most real-time PCR instruments other than the LightCycler 2.0 instruments. Available in two formulations, one contains the ROX reference dye and one does not. In combination with a real-time PCR instrument, suitable PCR primers, and a hydrolysis probe, FastStart TaqMan Probe Master

allows very sensitive detection and quantification of defined DNA sequences. The FastStart TaqMan Probe Master works well in combination with the probes of the Universal ProbeLibrary of Roche Diagnostics (Basel, Switzerland), which developed the new mixes.

The FastStart SYBR Green Master is a ready-to-use double-concentrated master mix that contains all the necessary reagents (except primers and template) and is suitable for quantitative real-time DNA detection assays whenever qPCR and two-step qRT-PCR assays in the SYBR Green I detection format are performed. In principle, the TaqMan Probe Master and the SYBR Green Master can be used for the amplification and detection of any DNA or cDNA target.