荷兰Kreatch公司推出新型核酸标记试剂盒

 
 
新型PlatinumBright核酸标记试剂盒具有知识产权的ULS（通用连接系统）标记分子，可以连接荧光染料或者半抗原，用于荧光原位杂交(FISH)实验。

完整的标记试剂盒包括通用纯化柱能够确保完全去除未反应的染料，并进行高质量的样本修复，而无论片段长度。ULS被进行工程改造，在实验方案之间与实验方案内部，提供能够高度复制的标记密度，而无需使用可能会导致偏性的酶。尽管是设计用于FISH的，但是标记反应同样可以用于Norhtern印迹、Southern印迹、条带印迹、点印迹杂交，以及组织分析。

ULS核酸标记是一种一步法操作，只需要30分钟。“PlatinumBright试剂盒的最终使用者能够非常简单、迅速而且可重复的标记核酸，只要他们能够想到的实验。”Kreatch生物工程公司(Amsterdam, The Netherlands; www.kreatech.com)商业应用部总经理、副总裁Brent Keller说。在今年的早些时候，该公司还投放了使用相同染料的一系列标记试剂盒，用于基因芯片（微阵列）对比基因组杂交分析的基因组DNA的直接标记。

Novel Nucleic Acid Labeling Kits
 
New PlatinumBright nucleic acid labeling kits offer a proprietary ULS (universal linkage system) labeling molecule bound to a choice of fluorescent dyes or haptens, for use in fluorescence in situ hybridization (FISH) applications.

The complete labeling kits include unique purification columns that ensure the complete removal of unreacted dye while yielding very high sample recovery, independent of fragment length. The ULS has been engineered to provide very reproducible labeling densities, both inter- and intra-experimental protocols, without the use of enzymes that can cause biases. While designed for FISH applications, the labeling reaction can also be used in Northern blot, Southern blot, spot blot, and dot blot hybridizations as well as with tissue arrays.

ULS labeling of nucleic acids is a one-step procedure that takes only 30 minutes. “End users using PlatinumBright kits can now very easily, quickly, and reproducibly label nucleic acids for whatever application they can think of,” observed Brent Keller, general manager/vice president, commercial operations, for Kreatech Biotechnology B.V. (Amsterdam, The Netherlands; www.kreatech.com). Earlier this year, the company also launched a line of labeling kits with the same dye pairs for the direct labeling of genomic DNA for microarray-based comparative genomic hybridization analysis.