Sangtec公司研制出一系列实时PCR诊断试剂盒

一系列即开即用型实时聚合酶链反应诊断试剂盒可以用于巨细胞病毒、乙肝病毒和EB病毒的DNA的定量检测。所有试剂盒都有CE标签。

所有试剂盒只需要体积很小(200 μl)的样本，具有敏感性高、动力范围广泛、检测协调性好，能够使得不同分析所需的多次运行在一次运行中即可完成。这些试剂盒叫做affigenea trender，是由Sangtec分子生物学诊断试剂公司研制开发的。用于检测巨细胞病毒和EB病毒的试剂盒可以用于血浆、血清和全血，而检测乙肝病毒的试剂盒只能用于血清和血浆。

Sangtec公司国际产品经理Therese Sundell指出：“affigenea trender试剂盒，为不同样本提供了灵活的相同的样本处理方案，比如不含细胞的体液和全血。试剂盒还通过设立内参照来防止出现假阴性的结果，通过UDG（尿嘧啶DNA糖基化酶）消毒以及封闭试管反应来防止出现假阳性结果。

Sangtec分子生物学诊断着眼于免疫减弱患者的感染性疾病和肿瘤学。该公司是世界上少数几家获得人类临床诊断PCR执照的公司之一。

Real-Time PCR Diagnostic Kits
 
A series of real-time polymerase chain reaction (PCR) diagnostic kits offer ready-to-use assays for DNA quantitation of cytomegalovirus (CMV), hepatitis B virus (HBV) and Epstein-Barr virus (EBV). All have the CE Mark.

All require only small sample volumes (200 μl) and are characterized by high sensitivity, wide dynamic range, and harmonized test profiles enabling multibatch runs with different analytes tested in one run. Called affigenea trender, the kits were developed by Sangtec Molecular Diagnostics (Stockholm, Sweden; www.sangtec.com). The assays for CMV and EBV are for use with plasma, scrum, and whole blood, while the HBV assay is for use in serum and plasma only.

“The affigenea trender kits offer a flexible solution for sample preparation from different sample matrices such as cell-free body fluids and whole blood,” noted Therese Sundell, international product manager at Sangtec. “They also offer prevention of false-negative results through a built-in internal control and prevention of false-positive results through UDG [uracil-DNA glycosylase] sterilization and closed-tube reactions.”

Sangtec Molecular Diagnostics focuses on infectious diseases of immunocompromised patients and oncology. The company states it is one of the few firms in the world with a broad PCR license for human clinical diagnostics.