使用外源性RNA参照物进行基因检测

 
临床和实验室标准研究院(CLSI, Wayne, PA, USA)指南提供了使用外源性RNA作为参照，主要用于基因芯片和定量实时PCR(QRT-PCR)为基础的基因表达。

外源性RNA参照指与需要分析检测样本中RNA不同的RNA。外援性RNA参照在例如逆转录和标记等酶促反应之前加入。该实验方案确保实验室、基因芯片和试剂生产商能够评估这些检测的性能。实验方案支持在基因芯片和QRT-PCR中使用外源性RNA。
 
Guidelines for Use of External RNA Controls
 
The Clinical and Laboratory Standards Institute (CLSI, Wayne, PA, USA) guidelines provide protocols for the use of external RNA controls in microarray and quantitative real-time polymerase chain reaction (QRT-PCR)-based gene expression.

External RNA controls refer to RNA species that are distinct from the RNAs in the sample that is to be analyzed. The external RNA controls are added prior to enzymatic manipulations, such as reverse transcription and labeling. The protocols enable laboratory, microarray, and reagent manufacturers to assess the performance of these expression assays. They support the use of external RNA controls in microarray and QRT-PCR-based gene expression experiments.